Ankita , Shukla and Sunil Kumar , Sanghi and V. , Sorna , Gowri and Vishal , , Kumar Baderia and Deepesh Kumar , Singh (2010) Determination of Biogenic Amines in Lake Water by Micellar Electrokinetic Chromatography with Fluorescence Detection after Derivatization with Fluorescamine1. Journal of Analytical Chemistry, 66. pp. 296-300. ISSN 1061-9348

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Abstract

A simple and rapid method has been developed for the determination of biogenic amines in lake water using micellar electrokinetic chromatography with fluorescence detection. Separation of fluorescam�ine derivatized biogenic amines was accomplished by using borate buffer of pH 9.5 containing 40 mM of sodium dodecyl sulphate. The method has been optimized with respect to fluorescamine concentration, reaction pH, reaction time, separation voltage and injection time. Detection was performed by using UG�11 excitation filter and 495 nm emission filter. The proposed method for histamine, tyramine and dopamine allowed their separation within 2 min with detection limits in nM range. The interday and intraday reproduc� ibility of peak areas were less than 6.5%. Recovery of spiked samples was 95.76–116.31%.

Item Type:	Article
Subjects:	Lightweight Materials
Divisions:	UNSPECIFIED
Depositing User:	Mr. B.K. Prasad
Date Deposited:	14 Aug 2013 08:53
Last Modified:	14 Aug 2013 08:53
URI:	http://ampri.csircentral.net/id/eprint/376

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