Joshi, P and Sanghi, S.K. and Kundu, S (2010) Immobilization of Monoamine Oxidase on Eggshell Membrane and Its Application in Designing an Amperometric Biosensor for Dopamine. Microchimica Acta. ISSN 0026-3672

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Abstract

An amperometric biosensor for dopamine is described. It is based on the enzyme monoamine oxidase immobilized on a glutaraldehyde-activated eggshell membrane that was deposited on a glassy carbon electrode. The Michaelis-Menten constant (Km) is 0.087 mM. Optimum pH and temperature conditions were obtained at pH 7.0 and 37 °C, respectively. The sensor showed a detection limit of 20 μM, a linear range from 50 μM to 250 μM, and a storage stability of ~25 days. In order to further improve the performance, a Nafion coating was applied on the electrode surface which gave favorable results with respect to shelf life of the enzyme (~40 days), the limit of detection, and the selectivity over ascorbic acid and uric acid.

Item Type:	Article
Subjects:	Waste-to-Wealth > Recovery from Waste
Divisions:	UNSPECIFIED
Depositing User:	Mr. B.K. Prasad
Date Deposited:	08 Jan 2014 08:00
Last Modified:	27 Jan 2014 07:38
URI:	http://ampri.csircentral.net/id/eprint/872

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